欧美午夜精品一区二区蜜桃,亚洲日韩乱码久久久久久,农村熟妇高潮精品A片,欧美一区二区三区免费A片

歡迎來到凱學生物科技(上海)有限公司網(wǎng)站!

熱門關鍵詞:進口ELISA試劑盒,人ELISA試劑盒,大鼠elisa試劑盒價格,小鼠elisa試劑盒價格,豬elisa試劑盒,雞elisa試劑盒,兔elisa試劑盒,魚elsa試劑盒,其他種屬elisa試劑盒,豚鼠elisa試劑盒,倉鼠elisa試劑盒,裸鼠ELISA試劑盒,進口試劑,血清,動物血清,人血清,胎牛血清,氨基酸試劑,培養(yǎng)基,顯色培養(yǎng)基,大腸桿菌O157培養(yǎng)基,細菌總數(shù)培養(yǎng)基,金黃色葡萄球菌檢驗培養(yǎng)基,沙門氏菌/賀氏菌檢驗培養(yǎng)基, 弧菌檢驗培養(yǎng)基,其他培養(yǎng)基,酵母 霉菌 青霉 曲霉培養(yǎng)基, 李斯特氏菌檢驗培養(yǎng)基,抗體,二抗,一抗,生物試劑,酶生物試劑,蛋白質(zhì)試劑,抗生素試劑,植物激素及核酸試劑,碳水化合物試劑,色素試劑,維生素試劑,表面活性劑,緩沖試劑,其他生化試劑,標準品對照品類,對照品,對照藥材,標準品,標準試劑,Spectrum試劑,美國藥典級試劑等

產(chǎn)品中心您現(xiàn)在的位置:首頁 > 產(chǎn)品展示 > ELISA試劑盒 > 人ELISA試劑盒 > 銷售人B細胞分化因子(BCDF)ELISA試劑盒

人B細胞分化因子(BCDF)ELISA試劑盒

簡要描述:

人B細胞分化因子(BCDF)ELISA試劑盒
使用目的:
為定量(性)測定活化的目標濃度的血清,血漿,組織勻漿,細胞培養(yǎng)上清及其他生物液體。研究使用不是用于診斷或治療程序。
如果你有任何問題,請上海義森生物科技有限公司。

更新時間:2024-09-07

 

人B細胞分化因子(BCDF)ELISA試劑盒

Reagent Kit.

120 times concentrated washing solution 20ml × 1 3ml× 1 bottles of 7 terminated liquid bottle

The 2 enzyme reagent 3ml ×1 bottles of 8 standard 960 μ g/L 0.5ml ×1 bottles

The 3 enzyme encapsulated plate 12 holes x 4 9 standard dilution of 1.5ml ×1 bottles

4 sample dilution 3ml ×1 bottles of 10 Manual 1

5 chromogenic reagent A fluid 3ml×1 bottles of 11 sealing plate film 2

6 chromogenic reagent B 3ml×12 1/ bottles of liquid sealing bag 1

Specimen requirements

1 specimens were collected after early extraction, extraction according to the related literature, after extracting the experiment should be conducted as soon as possible. If not immediay put the sample to test, can be stored at -20 ° C, but should avoid repeated freezing and thawing

2 could not be detected in the samples with NaN3, because NaN3 inhibition of horseradish peroxidase HRP activity.

Operation steps

Standard dilution: the kit provides raw times standard a, the user can according to the following chart in the small test tube dilution.

480 μ g/L 5 standard 150μl of the original times standard added 150 μ l standard dilution buffer

240 μ g/L 4 standard 150μL 5 standard added 150 μ l standard dilution buffer

120 μ g/L 3 standard 150μL 4 standard added 150 μ l standard dilution buffer

60 μ g/L 2 standard 150μL 3 standard added 150 μ l standard dilution buffer

30 μ g/L 1 standard 150μL 2 standard added 150 μ l standard dilution buffer

Sample: blank hole are respectively arranged blank hole without sample and enzyme reagent, the remaining steps the same, standard orifice, sample hole. In the enzyme coated board standard accurate sample adding 50 μ L, sample hole Zhongxian sample dilution of 40 μ L, then add the sample of 10μ L final dilution of the sample for 5 times). Sample sample on the enzyme labeled plate hole bottom, try not to touch the wall holes, gently shake mix.

Incubation: sealing plate membrane sealing plate is 37 ℃incubation for 30 minutes.

Liquid : 20 times concentrated washing solution with distilled water after 20 times dilution standby

Washing: carefully off the sealing plate membrane, abandoned to the liquid, drying, each hole is filled with a washing liquid, standing for 30 seconds after discarding, so repeated 5 times, pat dry.

 

 

Enzyme: each hole joining enzyme reagent 50 μ L, with the exception of blank hole.

Incubation: operation with 3.

Washing: operation with 5.

Color: each hole to join the chromogenic reagent A50μ L, adding chromogenic reagent B50μ L, gently shake mix, 37 ℃light color for 15 minutes.

Termination: each hole and terminated liquid 50μ L, termination reaction the blue vertical turn yellow.

Determination of air conditioning: with blank zero, 450nm wavelength in order to measure the absorbance OD) hole. Determination should be in plus termination solution within 15 minutes after the for.

Calculation

With a standard concentration as abscissa, OD as ordinate, on coordinate paper draw the standard curve, according to samples by the standard curve was found corresponding concentration; multiplied by the dilution factor; or standard concentration was calculated with standard curve equation of linear regression, sample od into the equation, calculation out of sample concentration, multiplied by the dilution factor, namely the actual sample concentration.

Matters needing attention

The 1 kit from refrigerated removed should be at room temperature balance 15-30 minutes before use, enzyme encapsulated plate in Kaifeng such as unused, slats into sealed bag should be stored in.

2 concentrated washing solution may crystallization, when diluted in water bath heating dissolution, washing does not affect the result.

3 step sampling shall be use the sample injector, and often to check that the accuracy, in order to avoid the test error. A sample time best control in 5 minutes, such as specimen number, recommended the use of volley sample.

人B細胞分化因子(BCDF)ELISA試劑盒

Please each measurement and standard curve, the best do complex holes. If the specimen substance to be determined in the content is too high sample od than standard hole of the first hole OD), please use the sample dilution dilution of certain multiple n times) after the measurement, calculation when you finally multiplied by the total dilution× n ×5).

Sealing plate membrane only one-time use, to avoid cross contamination.

The 6 substrate please keep in dark place.

7 strictly in accordance with the manual operation, test results must be with the enzyme mark instrument readings shall prevail.

8 of all samples, the washing liquid and a variety of waste should be according to the infection treatment.

The 9 different reagent batch components not mix.

10 such as with English instructions are different, with English instructions shall prevail.

Preservation conditions and period of validity

The 1 Kit: 2-8preservation.

The 2 period: 6 months

 

 

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯數(shù)字),如:三加四=7
当男朋友的面初次给了别人| 无码国内精品久久人妻| 久久精品国产WWW456C0M| 初尝人妻少妇中文字幕| 特黄做受又硬又粗又大视频小说| 欧美性做爰片免费视频看| 国产极品尤物粉嫩泬在线观看| 欧美人体艺术视频| 久久人人爽爽人人爽人人片AV| 老根嫩草1一40淑媛全文| 色噜噜精品一区二区三区| 一区二区三区在线 | 欧| 交换俱乐部娇妻奶呻吟啊视频| 国语自产少妇精品视频蜜桃在线| 久久久国产精品无码一区二区三区 | 国模无码视频一区二区三区| 部长侵犯部下属人妻A片| 少妇寂寞偷公乱400章深夜书屋 | 欧美日韩久久久精品A片| 精品人妻人人做人人爽夜夜爽| いっぱいしぼっちゃうぞ在线| 全职法师第6季全集免费观看| 永久免费AV无码国产网站18禁| 亚洲永久无码7777KKK| 歪歪漫画入口弹窗页面打开| 中文字幕精品久久久乱码乱码| 扒开双腿抽打花蒂惩罚室| 77777亚洲午夜久久多喷 | 小雪张开双腿给老赵上| 和上司出差被内谢在线播放| 精品亚洲成A人无码成A在线观看 | 亚洲国产精品无码AAA片| 最近免费观看高清韩国日本大全 | 国产韩国日本欧美的品牌SUV| 熟女精品视频一区二区三区| 天天摸天天做天天爽天天弄| 推特怎么在国内使用| 少妇性夜夜春夜夜爽A片| 国产激情精品一区二区三区 | 40厘米黑人与中国女人| 国语自产拍精品香蕉在线播放|